Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
Identifieur interne : 000868 ( Main/Exploration ); précédent : 000867; suivant : 000869Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
Auteurs : B. Radhika [Inde] ; N. Vinod Kumar [Inde] ; D. Sreenivasulu [Inde]Source :
- Veterinary World [ 0972-8988 ] ; 2016.
Abstract
The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of
A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of
Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of
Both LAMP and PCR for detection of
Url:
DOI: 10.14202/vetworld.2016.60-64
PubMed: 27051186
PubMed Central: 4819352
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000049
- to stream Pmc, to step Curation: 000049
- to stream Pmc, to step Checkpoint: 000279
- to stream Ncbi, to step Merge: 000540
- to stream Ncbi, to step Curation: 000540
- to stream Ncbi, to step Checkpoint: 000540
- to stream Main, to step Merge: 000870
- to stream Main, to step Curation: 000868
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of <italic>Clostridium perfringens</italic> alpha toxin gene in lambs by loop mediated isothermal amplification</title><author><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27051186</idno><idno type="pmc">4819352</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819352</idno><idno type="RBID">PMC:4819352</idno><idno type="doi">10.14202/vetworld.2016.60-64</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000049</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000049</idno><idno type="wicri:Area/Pmc/Curation">000049</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000049</idno><idno type="wicri:Area/Pmc/Checkpoint">000279</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000279</idno><idno type="wicri:Area/Ncbi/Merge">000540</idno><idno type="wicri:Area/Ncbi/Curation">000540</idno><idno type="wicri:Area/Ncbi/Checkpoint">000540</idno><idno type="wicri:Area/Main/Merge">000870</idno><idno type="wicri:Area/Main/Curation">000868</idno><idno type="wicri:Area/Main/Exploration">000868</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of <italic>Clostridium perfringens</italic> alpha toxin gene in lambs by loop mediated isothermal amplification</title><author><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>State Level Diagnostic Laboratory, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Veterinary Microbiology, College of Veterinary Science, Tirupathi, Andhra Pradesh</wicri:regionArea><wicri:noRegion>Andhra Pradesh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Veterinary World</title><idno type="ISSN">0972-8988</idno><idno type="eISSN">2231-0916</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Aim:</title><p>The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of <italic>Clostridium perfringens</italic>.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of <italic>C. perfringens cpa</italic> gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme <italic>XmnI</italic> for alpha toxin gene.</p></sec><sec id="st3"><title>Results:</title><p>Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of <italic>cpa</italic> gene which indicated the equal sensitivity of both the tests. The enzyme produced single cut in 162 base pair amplified product of alpha toxin gene at 81 base pair resulting in a single band in gel electrophoresis.</p></sec><sec id="st4"><title>Conclusion:</title><p>Both LAMP and PCR for detection of <italic>cpa</italic> gene indicated the equal sensitivity of both the tests. Standardization of LAMP reaction for amplification of epsilon and beta toxin genes will help to identify the <italic>C. perfringens</italic> toxin types from the clinical samples. The test could be a suitable alternative to the PCR in detection of toxin types without the help of sophisticated machinery like thermal cycler. Considering its simplicity in operation and high sensitivity, there is the potential use of this technique in clinical diagnosis and surveillance of infectious diseases.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Uzal, F A" uniqKey="Uzal F">F.A Uzal</name></author><author><name sortKey="Plumb, J" uniqKey="Plumb J">J Plumb</name></author><author><name sortKey="Blackall, L L" uniqKey="Blackall L">L.L Blackall</name></author><author><name sortKey="O Oyle, D" uniqKey="O Oyle D">D O’Boyle</name></author><author><name sortKey="Kelly, W R" uniqKey="Kelly W">W.R Kelly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dray, T" uniqKey="Dray T">T Dray</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khairy, E M" uniqKey="Khairy E">E.M Khairy</name></author><author><name sortKey="Dorgham, S M" uniqKey="Dorgham S">S.M Dorgham</name></author><author><name sortKey="Bakry, M A" uniqKey="Bakry M">M.A Bakry</name></author><author><name sortKey="Hakim, A S" uniqKey="Hakim A">A.S Hakim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meer, R R" uniqKey="Meer R">R.R Meer</name></author><author><name sortKey="Songer, J G" uniqKey="Songer J">J.G Songer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vinod Kumar, N" uniqKey="Vinod Kumar N">N Vinod Kumar</name></author><author><name sortKey="Sreenivasulu, D" uniqKey="Sreenivasulu D">D Sreenivasulu</name></author><author><name sortKey="Reddy, Y N" uniqKey="Reddy Y">Y.N Reddy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name></author><author><name sortKey="Sannarangaiah, S" uniqKey="Sannarangaiah S">S Sannarangaiah</name></author><author><name sortKey="Dash, P K" uniqKey="Dash P">P.K Dash</name></author><author><name sortKey="Rao, P V L" uniqKey="Rao P">P.V.L Rao</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Okayama, H" uniqKey="Okayama H">H Okayama</name></author><author><name sortKey="Masubuchi, H" uniqKey="Masubuchi H">H Masubuchi</name></author><author><name sortKey="Yonekawa, T" uniqKey="Yonekawa T">T Yonekawa</name></author><author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name></author><author><name sortKey="Amino, N" uniqKey="Amino N">N Amino</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name></author><author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name></author><author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name></author><author><name sortKey="Ohtsuka, K" uniqKey="Ohtsuka K">K Ohtsuka</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name></author><author><name sortKey="Russel, D W" uniqKey="Russel D">D.W Russel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kamachi, K" uniqKey="Kamachi K">K Kamachi</name></author><author><name sortKey="Toyoizumi Ajisaka, H" uniqKey="Toyoizumi Ajisaka H">H Toyoizumi-Ajisaka</name></author><author><name sortKey="Toda, K" uniqKey="Toda K">K Toda</name></author><author><name sortKey="Soeung, S C" uniqKey="Soeung S">S.C Soeung</name></author><author><name sortKey="Sarath, S" uniqKey="Sarath S">S Sarath</name></author><author><name sortKey="Nareth, Y" uniqKey="Nareth Y">Y Nareth</name></author><author><name sortKey="Horiuchi, Y" uniqKey="Horiuchi Y">Y Horiuchi</name></author><author><name sortKey="Kojima, K" uniqKey="Kojima K">K Kojima</name></author><author><name sortKey="Takahashi, M" uniqKey="Takahashi M">M Takahashi</name></author><author><name sortKey="Arakawa, Y" uniqKey="Arakawa Y">Y Arakawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Das, A" uniqKey="Das A">A Das</name></author><author><name sortKey="Babiuk, S" uniqKey="Babiuk S">S Babiuk</name></author><author><name sortKey="Mclntosh, M T" uniqKey="Mclntosh M">M.T Mclntosh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaneko, K" uniqKey="Kaneko K">K Kaneko</name></author><author><name sortKey="Miyamoto, K" uniqKey="Miyamoto K">K Miyamoto</name></author><author><name sortKey="Mimura, K" uniqKey="Mimura K">K Mimura</name></author><author><name sortKey="Yumine, N" uniqKey="Yumine N">N Yumine</name></author><author><name sortKey="Utsunomiya, H" uniqKey="Utsunomiya H">H Utsunomiya</name></author><author><name sortKey="Akimoto, S" uniqKey="Akimoto S">S Akimoto</name></author><author><name sortKey="Mcclane, A B" uniqKey="Mcclane A">A.B McClane</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aryan, E" uniqKey="Aryan E">E Aryan</name></author><author><name sortKey="Makvandi, M" uniqKey="Makvandi M">M Makvandi</name></author><author><name sortKey="Farajzadeh, A" uniqKey="Farajzadeh A">A Farajzadeh</name></author><author><name sortKey="Huygen, K" uniqKey="Huygen K">K Huygen</name></author><author><name sortKey="Bifani, P" uniqKey="Bifani P">P Bifani</name></author><author><name sortKey="Mousavi, S" uniqKey="Mousavi S">S Mousavi</name></author><author><name sortKey="Fateh, A" uniqKey="Fateh A">A Fateh</name></author><author><name sortKey="Jelodar, A" uniqKey="Jelodar A">A Jelodar</name></author><author><name sortKey="Gouya, M" uniqKey="Gouya M">M Gouya</name></author><author><name sortKey="Romano, M" uniqKey="Romano M">M Romano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suzuki, R" uniqKey="Suzuki R">R Suzuki</name></author><author><name sortKey="Ihira, M" uniqKey="Ihira M">M Ihira</name></author><author><name sortKey="Enomoto, Y" uniqKey="Enomoto Y">Y Enomoto</name></author><author><name sortKey="Yano, H" uniqKey="Yano H">H Yano</name></author><author><name sortKey="Maruyama, F" uniqKey="Maruyama F">F Maruyama</name></author><author><name sortKey="Emi, N" uniqKey="Emi N">N Emi</name></author><author><name sortKey="Asano, Y" uniqKey="Asano Y">Y Asano</name></author><author><name sortKey="Yoshikawa, T" uniqKey="Yoshikawa T">T Yoshikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hong, T C" uniqKey="Hong T">T.C Hong</name></author><author><name sortKey="Mai, Q L" uniqKey="Mai Q">Q.L Mai</name></author><author><name sortKey="Cuong, D V" uniqKey="Cuong D">D.V Cuong</name></author><author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name></author><author><name sortKey="Minekawa, H" uniqKey="Minekawa H">H Minekawa</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Hasebe, F" uniqKey="Hasebe F">F Hasebe</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hara Kudo, Y" uniqKey="Hara Kudo Y">Y Hara Kudo</name></author><author><name sortKey="Yoshino, M" uniqKey="Yoshino M">M Yoshino</name></author><author><name sortKey="Kojima, T" uniqKey="Kojima T">T Kojima</name></author><author><name sortKey="Ikedo, M" uniqKey="Ikedo M">M Ikedo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Radhika, B" sort="Radhika, B" uniqKey="Radhika B" first="B." last="Radhika">B. Radhika</name></noRegion><name sortKey="Kumar, N Vinod" sort="Kumar, N Vinod" uniqKey="Kumar N" first="N. Vinod" last="Kumar">N. Vinod Kumar</name><name sortKey="Sreenivasulu, D" sort="Sreenivasulu, D" uniqKey="Sreenivasulu D" first="D." last="Sreenivasulu">D. Sreenivasulu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000868 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000868 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:4819352
|texte= Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27051186" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |